purification system thermo scientific 5400110 (Thermo Fisher)
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purification system thermo scientific 5400110
Purification System Thermo Scientific 5400110, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/purification+system+thermo+scientific+5400110/purification+system+thermo+scientific+5400110/pm39121862-175-121-129
Average 90 stars, based on 1 article reviews
Purification System Thermo Scientific 5400110, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/purification+system+thermo+scientific+5400110/purification+system+thermo+scientific+5400110/pm39121862-175-121-129
Average 90 stars, based on 1 article reviews
purification system thermo scientific 5400110 - by Bioz Stars,
2026-10
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Recombinant:Article Title: Genome editing using type I-E CRISPR-Cas3 in mice and rat zygotes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E.coli:Bacterial Strain JM109(DE3) Promega Cat#P9801 E. coli: MAX EfficiencyTM DH5a Competent Cells Thermo Fisher Scientific Cat#18258012 Chemicals, peptides, and recombinant proteins ATP TaKaRa Cat#4041 KSOM ARK Resource https://www.ark-resource.co.jp/ product_re/142/ M2 ARK Resource https://www.ark-resource.co.jp/ product_re/142/ PMSG ASKA Animal Health Co., Ltd. https://aska-animal.co.jp/products/ detail/husbandry/sero.html hCG ASKA Pharmaceutical Co., Ltd. https://www.aska-pharma.co.jp/ iryouiyaku/item/detail.php?id=302 Rat KSOM ARK Resource https://www.ark-resource.co.jp/ product_re/142/ Tks Gflex DNA Polymerase TaKaRa Cat#R060A KOD One PCR Master Mix -Blue- TOYOBO Cat#KMM-201 KOD -Multi & Epi- TOYOBO Cat#KME-101 Critical commercial assays mMESSAGE mMACHINE T7 Transcription Kit Invitrogen Cat#AM1344 MEGAshortscript T7 Transcription Kit Invitrogen Cat#AM1354 CleanCap Reagent AG TriLink Cat#N-7113-1 Monarch RNA Cleanup Kit NEB Cat#T2040L MagMAXTM DNA Multi-Sample Ultra 2.0 Kit Applied Biosystems Cat#A36570 KOD -Multi & Epi- TOYOBO Cat#KME-101 Ligation Sequencing Kit V14 Oxford Nanopore technologies Cat#SQK-LSK-114 Deposited data Raw data of Nanopore sequencing This paper PRJNA1114408 Mouse reference genome NCBI GRCm39, mm39 Genome Reference Consortium https://www.ncbi.nlm.nih.gov/datasets/ genome/GCF_000001635.27/ Rat reference genome NCBI mRatBN7.2, rn7 Genome Reference Consortium https://www.ncbi.nlm.nih.gov/datasets/ genome/GCF_015227675.2/ Experimental models: cell lines Human: HEK293T cells ATCC Cat#CRL-3216 Experimental models: organisms/strains Mouse: C57BL/6JJcl CLEA Japan Inc. https://www.clea-japan.com/products/ inbred/item_a0420 Mouse: Jcl:ICR CLEA Japan Inc. https://www.clea-japan.com/products/ outbred/item_a0340 Mouse: NOD.Cg-Prkdcscid Il2rgtm1Sug/ShiJic the Central Institute for Experimental Medicine and Life Science https://www.ciea.or.jp/en/laboratory_ animal/nog.html Rat: F344/Jcl CLEA Japan Inc. https://www.clea-japan.com/products/ inbred/item_a0470 Rat: Iar:Wistar-Imamichi Institute for Animal Reproduction https://www.iar.or.jp/gp_rat/wi_rat/index_ wi.html (Continued on next page) Cell Reports Methods 4, 100833, August 19, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for genotyping, see Table S5 This paper N/A Recombinant DNA pDonor: Rosa26-GFP-mCherryReplacement, see Table S5 This paper N/A pDonor: Rosa26-GFP-mCherryIntegration, see Table S5 This paper N/A pDonor: mc-Kit-V831C, see Table S5 This paper N/A Software and algorithms Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ DAJIN2 Kuno et al.22 https://github.com/akikuno/DAJIN2 cstag Kuno43 https://github.com/akikuno/cstag mappy Li et al., 2018 https://github.com/lh3/minimap2/tree/ master/python pysam Li et al.41 https://github.com/pysam-developers/ pysam IGV Robinson et al., 2011 https://igv.org/ GGGenome Provided by DBCLS in Japan https://GGGenome.dbcls.jp/ UCSC Genome Browser Karolchik et al.42 https://genome.ucsc.edu/ Other Microscope OLYMPUS SZ61 Microscope OLYMPUS IX73 Manipulator NARISHIGE MMO-4 Manipulator NARISHIGE MM-94 Microinjector NARISHIGE IM-12 Microinjector Eppendorf FemtoJet 4i Electroporator Nepa Gene Co., Ltd. Software:Article Title: Genome editing using type I-E CRISPR-Cas3 in mice and rat zygotes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E.coli:Bacterial Strain JM109(DE3) Promega Cat#P9801 E. coli: MAX EfficiencyTM DH5a Competent Cells Thermo Fisher Scientific Cat#18258012 Chemicals, peptides, and recombinant proteins ATP TaKaRa Cat#4041 KSOM ARK Resource https://www.ark-resource.co.jp/ product_re/142/ M2 ARK Resource https://www.ark-resource.co.jp/ product_re/142/ PMSG ASKA Animal Health Co., Ltd. https://aska-animal.co.jp/products/ detail/husbandry/sero.html hCG ASKA Pharmaceutical Co., Ltd. https://www.aska-pharma.co.jp/ iryouiyaku/item/detail.php?id=302 Rat KSOM ARK Resource https://www.ark-resource.co.jp/ product_re/142/ Tks Gflex DNA Polymerase TaKaRa Cat#R060A KOD One PCR Master Mix -Blue- TOYOBO Cat#KMM-201 KOD -Multi & Epi- TOYOBO Cat#KME-101 Critical commercial assays mMESSAGE mMACHINE T7 Transcription Kit Invitrogen Cat#AM1344 MEGAshortscript T7 Transcription Kit Invitrogen Cat#AM1354 CleanCap Reagent AG TriLink Cat#N-7113-1 Monarch RNA Cleanup Kit NEB Cat#T2040L MagMAXTM DNA Multi-Sample Ultra 2.0 Kit Applied Biosystems Cat#A36570 KOD -Multi & Epi- TOYOBO Cat#KME-101 Ligation Sequencing Kit V14 Oxford Nanopore technologies Cat#SQK-LSK-114 Deposited data Raw data of Nanopore sequencing This paper PRJNA1114408 Mouse reference genome NCBI GRCm39, mm39 Genome Reference Consortium https://www.ncbi.nlm.nih.gov/datasets/ genome/GCF_000001635.27/ Rat reference genome NCBI mRatBN7.2, rn7 Genome Reference Consortium https://www.ncbi.nlm.nih.gov/datasets/ genome/GCF_015227675.2/ Experimental models: cell lines Human: HEK293T cells ATCC Cat#CRL-3216 Experimental models: organisms/strains Mouse: C57BL/6JJcl CLEA Japan Inc. https://www.clea-japan.com/products/ inbred/item_a0420 Mouse: Jcl:ICR CLEA Japan Inc. https://www.clea-japan.com/products/ outbred/item_a0340 Mouse: NOD.Cg-Prkdcscid Il2rgtm1Sug/ShiJic the Central Institute for Experimental Medicine and Life Science https://www.ciea.or.jp/en/laboratory_ animal/nog.html Rat: F344/Jcl CLEA Japan Inc. https://www.clea-japan.com/products/ inbred/item_a0470 Rat: Iar:Wistar-Imamichi Institute for Animal Reproduction https://www.iar.or.jp/gp_rat/wi_rat/index_ wi.html (Continued on next page) Cell Reports Methods 4, 100833, August 19, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for genotyping, see Table S5 This paper N/A Recombinant DNA pDonor: Rosa26-GFP-mCherryReplacement, see Table S5 This paper N/A pDonor: Rosa26-GFP-mCherryIntegration, see Table S5 This paper N/A pDonor: mc-Kit-V831C, see Table S5 This paper N/A Software and algorithms Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ DAJIN2 Kuno et al.22 https://github.com/akikuno/DAJIN2 cstag Kuno43 https://github.com/akikuno/cstag mappy Li et al., 2018 https://github.com/lh3/minimap2/tree/ master/python pysam Li et al.41 https://github.com/pysam-developers/ pysam IGV Robinson et al., 2011 https://igv.org/ GGGenome Provided by DBCLS in Japan https://GGGenome.dbcls.jp/ UCSC Genome Browser Karolchik et al.42 https://genome.ucsc.edu/ Other Microscope OLYMPUS SZ61 Microscope OLYMPUS IX73 Manipulator NARISHIGE MMO-4 Manipulator NARISHIGE MM-94 Microinjector NARISHIGE IM-12 Microinjector Eppendorf FemtoJet 4i Electroporator Nepa Gene Co., Ltd. Microscopy:Article Title: Genome editing using type I-E CRISPR-Cas3 in mice and rat zygotes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E.coli:Bacterial Strain JM109(DE3) Promega Cat#P9801 E. coli: MAX EfficiencyTM DH5a Competent Cells Thermo Fisher Scientific Cat#18258012 Chemicals, peptides, and recombinant proteins ATP TaKaRa Cat#4041 KSOM ARK Resource https://www.ark-resource.co.jp/ product_re/142/ M2 ARK Resource https://www.ark-resource.co.jp/ product_re/142/ PMSG ASKA Animal Health Co., Ltd. https://aska-animal.co.jp/products/ detail/husbandry/sero.html hCG ASKA Pharmaceutical Co., Ltd. https://www.aska-pharma.co.jp/ iryouiyaku/item/detail.php?id=302 Rat KSOM ARK Resource https://www.ark-resource.co.jp/ product_re/142/ Tks Gflex DNA Polymerase TaKaRa Cat#R060A KOD One PCR Master Mix -Blue- TOYOBO Cat#KMM-201 KOD -Multi & Epi- TOYOBO Cat#KME-101 Critical commercial assays mMESSAGE mMACHINE T7 Transcription Kit Invitrogen Cat#AM1344 MEGAshortscript T7 Transcription Kit Invitrogen Cat#AM1354 CleanCap Reagent AG TriLink Cat#N-7113-1 Monarch RNA Cleanup Kit NEB Cat#T2040L MagMAXTM DNA Multi-Sample Ultra 2.0 Kit Applied Biosystems Cat#A36570 KOD -Multi & Epi- TOYOBO Cat#KME-101 Ligation Sequencing Kit V14 Oxford Nanopore technologies Cat#SQK-LSK-114 Deposited data Raw data of Nanopore sequencing This paper PRJNA1114408 Mouse reference genome NCBI GRCm39, mm39 Genome Reference Consortium https://www.ncbi.nlm.nih.gov/datasets/ genome/GCF_000001635.27/ Rat reference genome NCBI mRatBN7.2, rn7 Genome Reference Consortium https://www.ncbi.nlm.nih.gov/datasets/ genome/GCF_015227675.2/ Experimental models: cell lines Human: HEK293T cells ATCC Cat#CRL-3216 Experimental models: organisms/strains Mouse: C57BL/6JJcl CLEA Japan Inc. https://www.clea-japan.com/products/ inbred/item_a0420 Mouse: Jcl:ICR CLEA Japan Inc. https://www.clea-japan.com/products/ outbred/item_a0340 Mouse: NOD.Cg-Prkdcscid Il2rgtm1Sug/ShiJic the Central Institute for Experimental Medicine and Life Science https://www.ciea.or.jp/en/laboratory_ animal/nog.html Rat: F344/Jcl CLEA Japan Inc. https://www.clea-japan.com/products/ inbred/item_a0470 Rat: Iar:Wistar-Imamichi Institute for Animal Reproduction https://www.iar.or.jp/gp_rat/wi_rat/index_ wi.html (Continued on next page) Cell Reports Methods 4, 100833, August 19, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for genotyping, see Table S5 This paper N/A Recombinant DNA pDonor: Rosa26-GFP-mCherryReplacement, see Table S5 This paper N/A pDonor: Rosa26-GFP-mCherryIntegration, see Table S5 This paper N/A pDonor: mc-Kit-V831C, see Table S5 This paper N/A Software and algorithms Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ DAJIN2 Kuno et al.22 https://github.com/akikuno/DAJIN2 cstag Kuno43 https://github.com/akikuno/cstag mappy Li et al., 2018 https://github.com/lh3/minimap2/tree/ master/python pysam Li et al.41 https://github.com/pysam-developers/ pysam IGV Robinson et al., 2011 https://igv.org/ GGGenome Provided by DBCLS in Japan https://GGGenome.dbcls.jp/ UCSC Genome Browser Karolchik et al.42 https://genome.ucsc.edu/ Other Microscope OLYMPUS SZ61 Microscope OLYMPUS IX73 Manipulator NARISHIGE MMO-4 Manipulator NARISHIGE MM-94 Microinjector NARISHIGE IM-12 Microinjector Eppendorf FemtoJet 4i Electroporator Nepa Gene Co., Ltd. Purification:Article Title: Genome editing using type I-E CRISPR-Cas3 in mice and rat zygotes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E.coli:Bacterial Strain JM109(DE3) Promega Cat#P9801 E. coli: MAX EfficiencyTM DH5a Competent Cells Thermo Fisher Scientific Cat#18258012 Chemicals, peptides, and recombinant proteins ATP TaKaRa Cat#4041 KSOM ARK Resource https://www.ark-resource.co.jp/ product_re/142/ M2 ARK Resource https://www.ark-resource.co.jp/ product_re/142/ PMSG ASKA Animal Health Co., Ltd. https://aska-animal.co.jp/products/ detail/husbandry/sero.html hCG ASKA Pharmaceutical Co., Ltd. https://www.aska-pharma.co.jp/ iryouiyaku/item/detail.php?id=302 Rat KSOM ARK Resource https://www.ark-resource.co.jp/ product_re/142/ Tks Gflex DNA Polymerase TaKaRa Cat#R060A KOD One PCR Master Mix -Blue- TOYOBO Cat#KMM-201 KOD -Multi & Epi- TOYOBO Cat#KME-101 Critical commercial assays mMESSAGE mMACHINE T7 Transcription Kit Invitrogen Cat#AM1344 MEGAshortscript T7 Transcription Kit Invitrogen Cat#AM1354 CleanCap Reagent AG TriLink Cat#N-7113-1 Monarch RNA Cleanup Kit NEB Cat#T2040L MagMAXTM DNA Multi-Sample Ultra 2.0 Kit Applied Biosystems Cat#A36570 KOD -Multi & Epi- TOYOBO Cat#KME-101 Ligation Sequencing Kit V14 Oxford Nanopore technologies Cat#SQK-LSK-114 Deposited data Raw data of Nanopore sequencing This paper PRJNA1114408 Mouse reference genome NCBI GRCm39, mm39 Genome Reference Consortium https://www.ncbi.nlm.nih.gov/datasets/ genome/GCF_000001635.27/ Rat reference genome NCBI mRatBN7.2, rn7 Genome Reference Consortium https://www.ncbi.nlm.nih.gov/datasets/ genome/GCF_015227675.2/ Experimental models: cell lines Human: HEK293T cells ATCC Cat#CRL-3216 Experimental models: organisms/strains Mouse: C57BL/6JJcl CLEA Japan Inc. https://www.clea-japan.com/products/ inbred/item_a0420 Mouse: Jcl:ICR CLEA Japan Inc. https://www.clea-japan.com/products/ outbred/item_a0340 Mouse: NOD.Cg-Prkdcscid Il2rgtm1Sug/ShiJic the Central Institute for Experimental Medicine and Life Science https://www.ciea.or.jp/en/laboratory_ animal/nog.html Rat: F344/Jcl CLEA Japan Inc. https://www.clea-japan.com/products/ inbred/item_a0470 Rat: Iar:Wistar-Imamichi Institute for Animal Reproduction https://www.iar.or.jp/gp_rat/wi_rat/index_ wi.html (Continued on next page) Cell Reports Methods 4, 100833, August 19, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for genotyping, see Table S5 This paper N/A Recombinant DNA pDonor: Rosa26-GFP-mCherryReplacement, see Table S5 This paper N/A pDonor: Rosa26-GFP-mCherryIntegration, see Table S5 This paper N/A pDonor: mc-Kit-V831C, see Table S5 This paper N/A Software and algorithms Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ DAJIN2 Kuno et al.22 https://github.com/akikuno/DAJIN2 cstag Kuno43 https://github.com/akikuno/cstag mappy Li et al., 2018 https://github.com/lh3/minimap2/tree/ master/python pysam Li et al.41 https://github.com/pysam-developers/ pysam IGV Robinson et al., 2011 https://igv.org/ GGGenome Provided by DBCLS in Japan https://GGGenome.dbcls.jp/ UCSC Genome Browser Karolchik et al.42 https://genome.ucsc.edu/ Other Microscope OLYMPUS SZ61 Microscope OLYMPUS IX73 Manipulator NARISHIGE MMO-4 Manipulator NARISHIGE MM-94 Microinjector NARISHIGE IM-12 Microinjector Eppendorf FemtoJet 4i Electroporator Nepa Gene Co., Ltd. Imaging:Article Title: Genome editing using type I-E CRISPR-Cas3 in mice and rat zygotes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E.coli:Bacterial Strain JM109(DE3) Promega Cat#P9801 E. coli: MAX EfficiencyTM DH5a Competent Cells Thermo Fisher Scientific Cat#18258012 Chemicals, peptides, and recombinant proteins ATP TaKaRa Cat#4041 KSOM ARK Resource https://www.ark-resource.co.jp/ product_re/142/ M2 ARK Resource https://www.ark-resource.co.jp/ product_re/142/ PMSG ASKA Animal Health Co., Ltd. https://aska-animal.co.jp/products/ detail/husbandry/sero.html hCG ASKA Pharmaceutical Co., Ltd. https://www.aska-pharma.co.jp/ iryouiyaku/item/detail.php?id=302 Rat KSOM ARK Resource https://www.ark-resource.co.jp/ product_re/142/ Tks Gflex DNA Polymerase TaKaRa Cat#R060A KOD One PCR Master Mix -Blue- TOYOBO Cat#KMM-201 KOD -Multi & Epi- TOYOBO Cat#KME-101 Critical commercial assays mMESSAGE mMACHINE T7 Transcription Kit Invitrogen Cat#AM1344 MEGAshortscript T7 Transcription Kit Invitrogen Cat#AM1354 CleanCap Reagent AG TriLink Cat#N-7113-1 Monarch RNA Cleanup Kit NEB Cat#T2040L MagMAXTM DNA Multi-Sample Ultra 2.0 Kit Applied Biosystems Cat#A36570 KOD -Multi & Epi- TOYOBO Cat#KME-101 Ligation Sequencing Kit V14 Oxford Nanopore technologies Cat#SQK-LSK-114 Deposited data Raw data of Nanopore sequencing This paper PRJNA1114408 Mouse reference genome NCBI GRCm39, mm39 Genome Reference Consortium https://www.ncbi.nlm.nih.gov/datasets/ genome/GCF_000001635.27/ Rat reference genome NCBI mRatBN7.2, rn7 Genome Reference Consortium https://www.ncbi.nlm.nih.gov/datasets/ genome/GCF_015227675.2/ Experimental models: cell lines Human: HEK293T cells ATCC Cat#CRL-3216 Experimental models: organisms/strains Mouse: C57BL/6JJcl CLEA Japan Inc. https://www.clea-japan.com/products/ inbred/item_a0420 Mouse: Jcl:ICR CLEA Japan Inc. https://www.clea-japan.com/products/ outbred/item_a0340 Mouse: NOD.Cg-Prkdcscid Il2rgtm1Sug/ShiJic the Central Institute for Experimental Medicine and Life Science https://www.ciea.or.jp/en/laboratory_ animal/nog.html Rat: F344/Jcl CLEA Japan Inc. https://www.clea-japan.com/products/ inbred/item_a0470 Rat: Iar:Wistar-Imamichi Institute for Animal Reproduction https://www.iar.or.jp/gp_rat/wi_rat/index_ wi.html (Continued on next page) Cell Reports Methods 4, 100833, August 19, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for genotyping, see Table S5 This paper N/A Recombinant DNA pDonor: Rosa26-GFP-mCherryReplacement, see Table S5 This paper N/A pDonor: Rosa26-GFP-mCherryIntegration, see Table S5 This paper N/A pDonor: mc-Kit-V831C, see Table S5 This paper N/A Software and algorithms Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ DAJIN2 Kuno et al.22 https://github.com/akikuno/DAJIN2 cstag Kuno43 https://github.com/akikuno/cstag mappy Li et al., 2018 https://github.com/lh3/minimap2/tree/ master/python pysam Li et al.41 https://github.com/pysam-developers/ pysam IGV Robinson et al., 2011 https://igv.org/ GGGenome Provided by DBCLS in Japan https://GGGenome.dbcls.jp/ UCSC Genome Browser Karolchik et al.42 https://genome.ucsc.edu/ Other Microscope OLYMPUS SZ61 Microscope OLYMPUS IX73 Manipulator NARISHIGE MMO-4 Manipulator NARISHIGE MM-94 Microinjector NARISHIGE IM-12 Microinjector Eppendorf FemtoJet 4i Electroporator Nepa Gene Co., Ltd. |